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Note however that depending on as well as the distribution of colours in the palette, there may not always be an exact solution for any given . Instead we’ll say that we want to minimise the absolute error between and some linear combination , or .

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Guar gum, a plant colloid, costs less than agar and is better suited for growing thermophilic bacteria, but is also more difficult to handle, being more viscous and less transparent. The bacterial polysaccharide xanthan is cheaper as well but forms weaker jellies that, as with carrageenan, might result in puncturing its surface. Other colloids, like alginate (from brown seaweed) and gellan gum (from a bacterium), don’t set solely based on temperature and require additives for gelation. These additives might interfere with microbial growth and make the preparation of those jellies less handy than agar plates.

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By WWII, scientists had already begun looking at alternative gelling substances for routine use in bacteriology, but concluded that agar was still better as it is both firmer and easier to handle. Today, some specialized microbiology applications use the colloid carrageenan (extracted from red seaweed Chondrus crispus, or “Irish Moss”), a more transparent and less auto-fluorescent alternative to agar (agar emits its own background fluorescence when excited by light). However, for routine bacteriological use, carrageenan is more difficult to dissolve, requires higher concentrations, can degrade at high temperatures, and forms weaker gels, which may result in puncturing its surface during the plating of cells.,更多细节参见91视频